The flora of Jhok Reserve Forest, a protected area near Lahore, Pakistan was surveyed for its ethno-medicinal potential. Forty-one plant species were found as ethno-botanically important for the local communities who utilize them in traditional medicine and practice. Many of these plants are also reported as common weeds, of which some are considered invasive alien species. Elders in the locality were interviewed about the utilization of these plant species growing in the reserve forest using a questionnaire approach. Out of 41 plant species (22 families) documented, 13 were those where all plant parts (roots, stems, leaves, flowers and seeds) were used in curing different ailments while for others one or more parts used. Some plants were described for their unique uses, for instance, the latex of Calotropis procera Aiton and Launaea procumbens Roxb. is used by local people for the treatment of toothache and skin infection, respectively.