The study was based on investigating the concentration of heavy metals in Parthenium hysterophorus sampled from the polluted locations of District Mardan and Nowshera, Khyber Pakhtunkhwa, Pakistan during 2015 in split plot design to ascertain its potential of heavy metal uptake and bioremediation of polluted soils. Samples of P. hysterophorus were collected from different Industrial, Agricultural and Dumping sites and were analyzed in the Soil and Environmental Sciences Laboratory at The University of Agriculture, Peshawar, Pakistan. The higher Cu content was observed in parthenium leaves (2.7 g/kg) while the lowest content was recorded in roots (2.4 g/kg). Highest Cu content was found in the industrial site (5.1g/kg), while the lowest observed in agriculture site. Highest Fe contents were observed in the stem (42.9 g/kg) and lowest were recorded in leaves (11.6 g/kg). The highest Fe content was found in dumping sites (38.3 g/kg) while lowest in agriculture sites (26.2 g/kg). Similarly the Highest Pb content was observed in stem (145 g/kg) while lowest in leaves (12.5 g/kg). High Pb amount was found in dumping site while lowest in agricultural site. Highest Ni content was observed in the stem (42.9 g/kg) while the lowest Ni content was recorded in leaves (11.4 g/kg). Similarly, High Ni amount was found in dumping site (39.2 g/kg) while lower in agricultural site (18.9 g/kg). Higher Cd contents were observed in the stem (42.9 g/kg) while the lowest in leaves (11.4 g/kg). Amongst sites, highest Cd amount was found in dumping site (39.2 g/kg) while lower in the agriculture site (18.9 g/kg). From the present study it is concluded that that dumping sites were more polluted in heavy metals as compared to industrial sites and the P. hysterophorus growing in highly polluted environments showed the ability of higher uptake and accumulation of different heavy metals in its different parts. This study, therefore, concluded that P. hysterophorus could be an option for bioremediation of highly polluted environments. These results need further confirmation of its potential of bioremediation by specifically growing it in pots with different heavy metal concentrations under contrrolled conditions.